Titania nanotube-modified screen printed carbon electrodes enhance the sensitivity in the electrochemical detection of proteins

Mandal, Soumit S. ; Navratna, Vikas ; Sharma, Pratyush ; Gopal, B. ; Bhattacharyya, Aninda J. (2014) Titania nanotube-modified screen printed carbon electrodes enhance the sensitivity in the electrochemical detection of proteins Bioelectrochemistry, 98 . pp. 46-52. ISSN 1567-5394

Full text not available from this repository.

Official URL: http://doi.org/10.1016/j.bioelechem.2014.03.003

Related URL: http://dx.doi.org/10.1016/j.bioelechem.2014.03.003

Abstract

The use of titania nanotubes (TiO2-NT) as the working electrode provides a substantial improvement in the electrochemical detection of proteins. A biosensor designed using this strategy provided a robust method to detect protein samples at very low concentrations (Cprotein ca 1 ng/μl). Reproducible measurements on protein samples at this concentration (Ip,a of 80 + 1.2 μA) could be achieved using a sample volume of ca 30 μl. We demonstrate the feasibility of this strategy for the accurate detection of penicillin binding protein, PBP2a, a marker for methicillin resistant Staphylococcus aureus (MRSA). The selectivity and efficiency of this sensor were also validated using other diverse protein preparations such as a recombinant protein tyrosine phosphatase (PTP10D) and bovine serum albumin (BSA). This electrochemical method also presents a substantial improvement in the time taken (few minutes) when compared to conventional enzyme-linked immunosorbent assay (ELISA) protocols. It is envisaged that this sensor could substantially aid in the rapid diagnosis of bacterial infections in resource strapped environments.

Item Type:Article
Source:Copyright of this article belongs to Elsevier B.V.
Keywords:Electrochemical Biosensor; Penicillin Binding Protein 2a; Methicillin Resistant Staphylococcus Aureus; Titania Nanotubes.
ID Code:116822
Deposited On:09 Apr 2021 09:33
Last Modified:09 Apr 2021 09:33

Repository Staff Only: item control page