Balasubramanian, A. S. ; Spolter, L. ; Rice, L. I. ; Sharon, J. B. ; Marx, W. (1967) Preparation of 3'-phosphoadenylyl sulfate in substrate quantities using mastocytoma enzymes Analytical Biochemistry, 21 (1). pp. 22-23. ISSN 0003-2697
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000326...
Related URL: http://dx.doi.org/10.1016/0003-2697(67)90078-4
Abstract
A method was developed for the enzymic preparation and purification of 3'-phosphoadenylyl sulfate labeled with radioactive sulfur. The proposed procedure includes incubation of mouse mast cell tumor (Furth) high-speed supernatant as a source of sulfate-activating enzymes with ATP and sulfate-35S in the presence of an ATP-generating system and other cofactors. Experimental conditions were established for maximal production of the active sulfate. The radioactive 3'-phosphoadenylyl sulfate synthesized was isolated by ion-exchange column chromatography on ECTEOLA formate. The ammonium bicarbonate used as eluent was removed by lyophilization.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 1165 |
| Deposited On: | 05 Oct 2010 12:49 |
| Last Modified: | 13 May 2011 06:46 |
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