Kaushik, Deepak Kumar ; Basu, Anirban (2013) Microglial Activation: Measurement of Cytokines by Flow Cytometry Microglia, 1041 . pp. 71-82. ISSN 1064-3745
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Official URL: http://doi.org/10.1007/978-1-62703-520-0_9
Related URL: http://dx.doi.org/10.1007/978-1-62703-520-0_9
Abstract
Cytokine measurement is a prerequisite to understand the inflammatory state of the body. Quantitative analysis of cytokines by Western blotting and ELISA is a daunting task as these are time-consuming and error-prone protocols. With the advent of flow cytometry, the estimation of cytokines using the classical antigen–antibody reaction has become a popular choice with researchers/clinicians. Here, we describe a protocol for multiple cytokine analysis using flow cytometry.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer Nature Switzerland AG. |
| Keywords: | Microglia; Cytokines; Cytokine bead array; Flow cytometry; Inflammation. |
| ID Code: | 115594 |
| Deposited On: | 18 Mar 2021 04:09 |
| Last Modified: | 18 Mar 2021 04:09 |
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