Leader RNA binding ability of chandipura virus P protein is regulated by its phosphorylation status: a possible role in genome transcription-replication switch

Basak, Soumen ; Raha, Tamal ; Chattopadhyay, Debasish ; Majumder, Amitabha ; Shaila, M.S ; Chattopadhyay, D.J (2003) Leader RNA binding ability of chandipura virus P protein is regulated by its phosphorylation status: a possible role in genome transcription-replication switch Virology, 307 (2). pp. 372-385. ISSN 0042-6822

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Official URL: http://doi.org/10.1016/S0042-6822(02)00093-4

Related URL: http://dx.doi.org/10.1016/S0042-6822(02)00093-4

Abstract

The molecular events associated with the transcriptive and replicative cycle of negative-stranded RNA viruses are still an enigma. We took Chandipura virus, a member of the Rhabdoviridae family, as our model system to demonstrate that Phosphoprotein P, besides Nucleocapsid protein N, also acts as a leader RNA-binding protein in its unphosphorylated form, whereas CKII-mediated phosphorylation totally abrogates its RNA-binding ability. However, interaction between P protein and leader RNA can be distinguished from N-mediated encapsidation of viral sequences. Furthermore, P protein bound to leader chain can successively recruit N protein on RNA while itself being replaced. We also observed that the accumulation of phosphorylation null mutant of P protein in cells results in enhanced genome RNA replication with concurrent increase in the viral yield. All these results led us to propose a model explaining viral transcription-replication switch where Phosphoprotein P acts as a modulator of genome transcription and replication by its ability to bind to the nascent leader RNA in its unphosphorylated form, promoting read-through of the transcription termination signals and initiating nucleocapsid assembly on the nascent RNA chain.

Item Type:Article
Source:Copyright of this article belongs to Elsevier B.V.
Keywords:Leader RNA; Phosphoprotein; Transcription; Replication; Chandipura Virus.
ID Code:115238
Deposited On:17 Mar 2021 04:24
Last Modified:17 Mar 2021 04:24

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