Exogenous delivery of a pyrazole based bioactive probe to natural DNA through non-ionic TX-165 micellar carrier

Abstract

In this article, we have reported the exogenous delivery of a newly synthesized biologically relevant fluorophore, namely, (E)-1,5-diphenyl-3-styryl-4,5 -dihydro-1H-pyrazole (DSDP) from a non-ionic Triton X-165 micellar nanocarrier to the biomolecular target DNA, exploiting an external stimulant β-cyclodextrin. Vivid steady state and time resolved spectroscopic studies reveal that in the presence of both the micelle and the ctDNA, the probe remains exclusively within the micellar environment because of the higher binding affinity of the probe with the former. However, upon addition of β-CD, the probe is displaced from the micellar environment to get bound to the DNA grooves. Inclusion complexation property of the stimulant with the hydrophobic chain of the constituents of the micelle is assigned to be responsible for the transfer of the probe from the micellar carrier to the DNA. Non-toxic biocompatible micellar carrier and cyclodextrin of proper dimension may be exploited to execute the present strategy of exogenous activation for effective and targeted delivery of drugs.