Inhibition of a transcriptional pause by RNA Anchoring to RNA polymerase

Komissarova, Natalia ; Velikodvorskaya, Tatiana ; Sen, Ranjan ; King, Rodney A. ; Banik-Maiti, Sarbani ; Weisberg, Robert A. (2008) Inhibition of a transcriptional pause by RNA Anchoring to RNA polymerase Molecular Cell, 31 (5). pp. 683-694. ISSN 1097-2765

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Official URL: https://www.cell.com/molecular-cell/fulltext/S1097...

Related URL: http://dx.doi.org/10.1016/j.molcel.2008.06.019

Abstract

We describe a mechanism by which nascent RNA inhibits transcriptional pausing. PutL RNA of bacteriophage HK022 suppresses transcription termination at downstream terminators and pausing within a nearby U-rich sequence. In vitro transcription and footprinting assays reveal that this pausing results from backtracking of RNA polymerase and that binding of nascent putL RNA to polymerase limits backtracking by restricting re-entry of the transcript into the RNA exit channel. The restriction is local and relaxes as the transcript elongates. Our results suggest that putL RNA binds to the surface of polymerase close to the RNA exit channel, a region that includes amino acid residues important for antitermination. Although binding is essential for antipausing and antitermination, these two activities of put differ: antipausing is limited to the immediate vicinity of the putL site, but antitermination is not. We propose that RNA anchoring to the elongation complex is a widespread mechanism of pause regulation.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:114776
Deposited On:04 Jun 2018 06:16
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