A multipronged strategy of an anti-terminator protein to overcome Rho-dependent transcription termination

Muteeb, Ghazala ; Dey, Debashish ; Mishra, Saurabh ; Sen, Ranjan (2012) A multipronged strategy of an anti-terminator protein to overcome Rho-dependent transcription termination Nucleic Acids Research, 40 (22). pp. 11213-11228. ISSN 0305-1048

[img]
Preview
PDF - Publisher Version
8MB

Official URL: https://academic.oup.com/nar/article/40/22/11213/1...

Related URL: http://dx.doi.org/10.1093/nar/gks872

Abstract

One of the important role of Rho-dependent transcription termination in bacteria is to prevent gene expressions from the bacteriophage DNA. The transcription anti-termination systems of the lambdoid phages have been designed to overcome this Rho action. The anti-terminator protein N has three interacting regions, which interact with the mRNA, with the NusA and with the RNA polymerase. Here, we show that N uses all these interaction modules to overcome the Rho action. N and Rho co-occupy their overlapping binding sites on the nascent RNA (the nutR/tR1 site) and this configuration slows down the rate of ATP hydrolysis and the rate of RNA release by Rho from the elongation complex. N-RNA polymerase interaction is not too important for this Rho inactivation process near/at the nutR site. This interaction becomes essential when the elongation complex moves away from the nutR site. From the unusual NusA-dependence property of a Rho mutant E134K, a suppressor of N, we deduced that the N-NusA complex in the anti-termination machinery reduces the efficiency of Rho by removing NusA from the termination pathway. We propose that NusA-remodelling is also one of the mechanisms used by N to overcome the termination signals.

Item Type:Article
Source:Copyright of this article belongs to Oxford University Press.
ID Code:114758
Deposited On:04 Jun 2018 07:53
Last Modified:04 Jun 2018 07:53

Repository Staff Only: item control page