Green tea polyphenols as inhibitors of ribonuclease A

Ghosh, Kalyan Sundar ; Maiti, Tushar Kanti ; Dasgupta, Swagata (2004) Green tea polyphenols as inhibitors of ribonuclease A Biochemical and Biophysical Research Communications, 325 (3). pp. 807-811. ISSN 0006-291X

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Ribonucleases (RNases), which are essential for cleavage of RNA, may be cytotoxic due to undesired cleavage of RNA in the cell. The quest for small molecule inhibitors of members of the ribonuclease superfamily has become indispensable with a growing number exhibiting unusual biological properties. Thus, inhibitors of RNases may serve as potential drug candidates. Green tea catechins (GTC), particularly its major constituent (−)-epigallocatechin-3-gallate (EGCG), have reported potential against cell proliferation and angiogenesis induced by several growth factors including angiogenin, a member of the RNase superfamily. This study reports the inhibition of bovine pancreatic ribonuclease A (RNase A) by EGCG and GTC. This has been checked qualitatively by an agarose gel based assay. Enzyme kinetic studies with cytidine 2′,3′ cyclic monophosphate as the substrate have also been conducted. Results indicate substantial inhibitory activity of a noncompetitive nature with an inhibition constant of ∼80 μM for EGCG and ∼100 μM for GTC measured in gallic acid equivalents.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Ribonuclease A; Green Tea Catechins; (−)-Epigallocatechin-3-Gallate; Agarose Gel Based Assay; Noncompetitive Inhibitor
ID Code:113761
Deposited On:15 May 2018 11:34
Last Modified:15 May 2018 11:34

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