The influence of common metal ions on the interactions of the isoflavone genistein with bovine serum albumin

Singha Roy, Atanu ; Tripathy, Debi Ranjan ; Chatterjee, Angshuman ; Dasgupta, Swagata (2013) The influence of common metal ions on the interactions of the isoflavone genistein with bovine serum albumin Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 102 . pp. 393-402. ISSN 1386-1425

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The interaction of genistein with bovine serum albumin (BSA) has been characterized via UV–vis, fluorescence spectroscopy and Circular Dichroism (CD) measurements under physiological conditions. In this study, we have investigated the effect of some common metal ions on the binding of genistein with BSA using fluorescence studies. The fluorescence data reveal that the binding affinity of genistein to BSA increases in presence of certain metal ions. The possibility of non-radiative energy transition from the donor tryptophan to the acceptor genistein has been observed in absence and presence of metal ions. The observed similarities in the values of efficiency of energy transfer (E) and the separation between the donor and acceptor (r) in both the cases may be correlated with the complexation between the genistein and metal ions, which is also observed from the UV–vis studies. The changes in enthalpy (ΔH°) and entropy (ΔS°) of the interaction were found to be −14.64 kJ mol−1 and +42.75 J mol−1 K−1 respectively. These values indicate the involvement of electrostatic interactions along with a hydrophobic association that results in a positive entropy change. CD analysis shows that there is a slight increase in the% α-helical content of BSA on binding with genistein at lower molar ratios. Warfarin and ibuprofen displacement studies in accordance with the molecular docking show that genistein binds to site I (subdomain IIA) of BSA.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Bovine Serum Albumin; Genistein; Metal Ions; UV–vis; Circular Dichroism; Fluorescence Quenching
ID Code:113439
Deposited On:10 May 2018 11:44
Last Modified:10 May 2018 11:44

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