Structural differences between native hen egg white lysozyme and its fibrils under different environmental conditions

Bhattacharya, Susmita ; Ghosh, Sudeshna ; Dasgupta, Swagata ; Roy, Anushree (2013) Structural differences between native hen egg white lysozyme and its fibrils under different environmental conditions Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 114 . pp. 368-376. ISSN 1386-1425

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Official URL: https://www.sciencedirect.com/science/article/pii/...

Related URL: http://dx.doi.org/10.1016/j.saa.2013.05.060

Abstract

The difference in molecular structure of native HEWL and its fibrils, grown at a pH value near physiological pH 7.4 and at a pH value just above the pI, 10.7 in presence and absence of Cu(II) ions, is discussed. We focus on differences between the molecular structure of the native protein and fibrils using principal component analysis of their Raman spectra. The overlap areas of the scores of each species are used to quantify the difference in the structure of the native HEWL and fibrils in different environments. The overall molecular structures are significantly different for fibrils grown at two pH values. However, in presence of Cu(II) ions, the fibrils have similarities in their molecular structures at these pH environments. Spectral variation within each species, as obtained from the standard deviations of the scores in PCA plots, reveals the variability in the structure within a particular species.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Fibrils; HEWL; Raman Scattering; Principal Component Analysis
ID Code:113427
Deposited On:10 May 2018 11:29
Last Modified:10 May 2018 11:29

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