MicroRNA 155 regulates Japanese Encephalitis Virus-Induced Inflammatory Response by targeting Src homology 2-containing inositol phosphatase 1

Thounaojam, Menaka Chanu ; Kundu, Kiran ; Kaushik, Deepak Kumar ; Swaroop, Shalini ; Mahadevan, Anita ; Shankar, Susarla Krishna ; Basu, Anirban (2014) MicroRNA 155 regulates Japanese Encephalitis Virus-Induced Inflammatory Response by targeting Src homology 2-containing inositol phosphatase 1 Journal of Virology, 88 (9). pp. 4798-4810. ISSN 0022-538X

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Official URL: http://jvi.asm.org/content/88/9/4798.full

Related URL: http://dx.doi.org/10.1128/JVI.02979-13

Abstract

MicroRNAs (miRNAs) are single-stranded small RNA molecules that regulate various cellular processes. miRNA 155 (miR-155) regulates various aspects of innate and adaptive immune responses and plays a key role in various viral infections and the resulting neuroinflammation. The present study evaluated the involvement of miR-155 in modulating Japanese encephalitis virus (JEV)-induced neuroinflammation. We observed that miR-155 expression was upregulated during JEV infection of mouse primary microglia, the BV-2 microglia cell line, and in both mouse and human brains. In vitro and in vivo knockdown of miR-155 minimized JEV-induced inflammatory responses. In the present study, we confirmed targeting of the Src homology 2-containing inositol phosphatase 1 (SHIP1) 3′ untranslated region (UTR) by miR-155 in the context of JEV infection. Inhibition of SHIP1 by miR-155 resulted in higher beta interferon (IFN-β) and proinflammatory cytokine production through activation of TANK-binding kinase 1 (TBK-1). Based on these observations, we conclude that miR-155 modulates the neuroinflammatory response during JEV infection via negative regulation of SHIP1 expression. Thus, modulation of miR-155 could be a novel strategy to regulate JEV-induced neuroinflammation.

Item Type:Article
Source:Copyright of this article belongs to American Society for Microbiology.
ID Code:113368
Deposited On:05 Jun 2018 09:43
Last Modified:05 Jun 2018 09:43

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