Gallic acid induced dose dependent inhibition of lysozyme fibrillation

Konar, Mouli ; Bag, Sudipta ; Roy, Pritam ; Dasgupta, Swagata (2017) Gallic acid induced dose dependent inhibition of lysozyme fibrillation International Journal of Biological Macromolecules, 103 . pp. 1224-1231. ISSN 0141-8130

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Amyloidosis is primarily characterized by the deposition of misfolded protein aggregates. Although the natural polyphenols have long been known as effective amyloid inhibitors, the mechanistic details of their inhibitory actions still remain unclear. Our present study explores the inhibition mechanism of polyphenols by studying the anti-amyloidogenic property of gallic acid (GA), the smallest structural unit of tea polyphenols, on hen egg white lysozyme (HEWL) at physiological pH. Using various spectroscopic techniques such as UV-vis, fluorescence, circular dichroism and dynamic light scattering, and microscopic techniques such as TEM and FESEM, it has been shown that GA potentially inhibits the self-aggregation process in a concentration dependent manner. Gel electrophoresis studies suggest that the o-dihydroxy moiety of GA is oxidized into the quinone moiety and H2O2 in the system under the experimental conditions. The quinone binds near the hydrophobic region of HEWL and restricts hydrophobic exposure. Cyclic voltammetry studies reveal that the Met residues of HEWL are oxidized by H2O2 to highly polar sulfoxide-modified side chains. The partially unfolded intermediates formed under the denaturing conditions employed remain in contact with the solvent thus preventing further aggregation.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Hen Egg White Lysozyme; Amyloid Fibril; Gallic Acid
ID Code:113313
Deposited On:15 May 2018 06:09
Last Modified:15 May 2018 06:09

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