Microfluorometric detection of catecholamines with multiphoton-excited fluorescence

Balaji, J. ; Reddy, Chandra S. ; Kaushalya, S. K. ; Maiti, Sudipta (2004) Microfluorometric detection of catecholamines with multiphoton-excited fluorescence Applied Optics, 43 (12). pp. 2412-2417. ISSN 0003-6935

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Official URL: https://www.osapublishing.org/ao/abstract.cfm?uri=...

Related URL: http://dx.doi.org/10.1364/AO.43.002412


We demonstrate sensitive spatially resolved detection of physiological chromophores that emit in the ultraviolet (<330 nm). An atypical laser source (a visible wavelength femtosecond optical parametric oscillator), and an unconventional collection geometry (a lensless detector that detects the forward-emitted fluorescence) enable this detection. We report the excitation spectra of the catecholamines dopamine and norepinephrine, together with near-UV emitters serotonin and tryptophan, in the range of 550–595 nm. We estimate the molecular two-photon action cross section of dopamine, norepinephrine, and serotonin to be 1.2 mGM (1 GM, or Goppert Mayor, is equal to 10-58 m4 s-1 photon-1), 2 mGM, and 43 mGM, respectively, at 560 nm. The sensitivity achieved by this method holds promise for the microscopic imaging of vesicular catecholamines in live cells.

Item Type:Article
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ID Code:113068
Deposited On:24 May 2018 11:45
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