Fluorescence correlation microscopy with real-time alignment readout

Kaushalya, Sanjeev Kumar ; Balaji, Jayaprakash ; Garai, Kanchan ; Maiti, Sudipta (2005) Fluorescence correlation microscopy with real-time alignment readout Applied Optics, 44 (16). pp. 3262-3265. ISSN 0003-6935

Full text not available from this repository.

Official URL: https://www.osapublishing.org/ao/abstract.cfm?uri=...

Related URL: http://dx.doi.org/10.1364/AO.44.003262


In confocal fluorescence correlation microscopy (FCM) it is important to ensure that the correlation measurement is actually performed at the chosen location of the three-dimensional image of the specimen. We present a confocal FCM design that provides an automatic real-time readout of the location in the confocal microscopic image, which is aligned with the detector of the fluorescence correlation spectrometer. The design accomplishes this without using any special positioning device. The design is based on an apertured fluorescence detector placed close to the back aperture of the objective lens and can be easily incorporated into virtually any confocal microscope. We demonstrate the method by performing FCM measurements of a dye diffusing on a cell membrane.

Item Type:Article
Source:Copyright of this article belongs to Optical Society of America.
ID Code:113066
Deposited On:24 May 2018 09:18
Last Modified:24 May 2018 09:18

Repository Staff Only: item control page