An insight into the ribonucleolytic and antiangiogenic activity of buffalo lactoferrin

Tripathy, Debi Ranjan ; Pandey, Nitin K. ; Dinda, Amit Kumar ; Ghosh, Sudeshna ; Roy, Atanu Singha ; Dasgupta, Swagata (2015) An insight into the ribonucleolytic and antiangiogenic activity of buffalo lactoferrin Journal of Biomolecular Structure & Dynamics, 33 (1). pp. 184-195. ISSN 0739-1102

Full text not available from this repository.

Official URL:

Related URL:


Lactoferrin (LF) has several biological effects ranging from ribonuclease activity to antiangiogenic activity. It thus serves as a potential target protein for studies related to ribonucleolytic activity in association with its antiangiogenic activity. We have isolated buffalo LF and checked the ribonucleolytic activity via an agarose gel-based assay and precipitation assay. The ribonucleolytic activity of LF is lower compared to RNase A and the pH profile is a bell-shaped curve, with a pK1 value of 5.43 and pK2 of 7.65. The ribonuclease inhibitor that inhibits many ribonuclease-type proteins by forming a tight complex is unable to inhibit the ribonucleolytic property of LF. Fe(III) behaves as a noncompetitive inhibitor for the ribonucleolytic activity of protein. The superoxide-scavenging activity of the protein has also been measured. Histidine modification by diethylpyrocarbonate was monitored by UV–Vis spectroscopy at pH 7 and pH 8 and the effect towards the ribonucleolytic activity was determined. The antiangiogenic property of LF was investigated by the chorioallantoic membrane assay. Finally, the possible active site was analyzed via docking studies and correlated with the experimental study.

Item Type:Article
Source:Copyright of this article belongs to Taylor and Francis Group.
Keywords:Buffalo Lactoferrin; Ribonuclease Inhibitor; Ribonucleolytic Property; Anti Angiogenic Property; Superoxide-Scavenging Property
ID Code:112947
Deposited On:10 May 2018 11:22
Last Modified:10 May 2018 11:22

Repository Staff Only: item control page