Isolation and partial characterization of ribonuclease inhibitor from goat liver

Chatterjee, Juin ; Maiti, Tushar Kanti ; Dasgupta, Swagata (2006) Isolation and partial characterization of ribonuclease inhibitor from goat liver Protein and Peptide Letters, 13 (8). pp. 779-783. ISSN 0929-8665

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Official URL: http://www.eurekaselect.com/57387/article

Related URL: http://dx.doi.org/10.2174/092986606777841307

Abstract

Ribonuclease inhibitor (RI), a 50 kDa protein, has been found both in mammalian and nonmammalian tissues. We have isolated RI from goat liver and partial characterization has been accomplished. For the isolation of RI, DEAE cellulose column chromatography followed by affinity chromatography using CNBr activated Sepharose 4B was performed. The inhibition of ribonucleolytic activity of Ribonuclease A has been checked by an agarose gel based assay. The antiangiogenic property of the protein was tested by the chorioallantoic membrane (CAM) assay. Results indicate inhibition of angiogenesis.

Item Type:Article
Source:Copyright of this article belongs to Bentham Science Publishers.
Keywords:Ribonuclease Inhibitor; Agarose Gel Based Assay; Angiogenesis; CAM Assay
ID Code:112890
Deposited On:15 May 2018 10:00
Last Modified:15 May 2018 10:00

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