Effect of temperature and solvent on fibrillation of human serum albumin

Pandey, Nitin K. ; Ghosh, Sudeshna ; Tripathy, Debi R. ; Dasgupta, Swagata (2015) Effect of temperature and solvent on fibrillation of human serum albumin Protein and Peptide Letters, 22 (2). pp. 112-118. ISSN 0929-8665

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Official URL: http://www.eurekaselect.com/121053/article

Related URL: http://dx.doi.org/10.2174/0929866521666140320104409


During the past several years, studies on the protein aggregation process in the presence of cosolvents/ co-solutes have been looked into which provides significant insight in the stability of proteins in a crowded cellular milieu. Here, in the present report we have investigated the fibrillation of human serum albumin (HSA) under the mixed aqueous-ethanol solvent conditions at two different temperatures (37 °C and 65 °C). Self-association of protein was monitored using various spectroscopic and microscopic techniques. Results obtained from detailed investigation have shown that fibrillation of human serum albumin is favored at higher temperature (65 °C) at lower ethanol concentration. However, at 37 °C comparatively higher ethanol concentration is the prerequisite condition for fibrillation process to take place.

Item Type:Article
Source:Copyright of this article belongs to Bentham Science Publishers.
Keywords:Ethanol; Fibrillation; Human Serum Albumin; Solvent; Spectroscopy; Temperature
ID Code:112867
Deposited On:15 May 2018 08:14
Last Modified:15 May 2018 08:14

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