Samaddar, Sandip ; Dutta, Ayan ; Sinharoy, Senjuti ; Paul, Anindita ; Bhattacharya, Avisek ; Saha, Sudip ; Chien, Ko-yi ; Goshe, Michael B. ; DasGupta, Maitrayee (2013) Autophosphorylation of gatekeeper tyrosine by symbiosis receptor kinase FEBS Letters, 587 (18). pp. 2972-2979. ISSN 0014-5793
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Official URL: https://www.sciencedirect.com/science/article/pii/...
Related URL: http://dx.doi.org/10.1016/j.febslet.2013.07.050
Abstract
Plant Receptor-like Kinases (RLKs) share their evolutionary origin with animal interleukin-1 Receptor-associated Kinase (IRAK)/Pelle family of soluble kinases and are distinguished by having tyrosine as ‘gatekeeper’. This position is adjacent to the hinge region and is hidden in a hydrophobic pocket of the catalytic cleft of protein kinases and is therefore least probable to be a target for any modification. This communication illustrates the accessibility of the gatekeeper site (Y670) towards both autophosphorylation and dephosphorylation in the recombinant cytoplasmic domain of symbiosis receptor kinase from Arachis hypogaea (AhSYMRK). Autophosphorylation on gatekeeper tyrosine was detected prior to extraction but never under in vitro conditions. We hypothesize gatekeeper phosphorylation to be associated with synthesis/maturation of AhSYMRK and this phenomenon may be prevalent among RLKs.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Autophosphorylation; Gatekeeper Residue; Receptor-like Kinase; Symbiosis Receptor Kinase; Tyrosine Kinase-like |
| ID Code: | 112452 |
| Deposited On: | 29 May 2018 06:11 |
| Last Modified: | 29 May 2018 06:11 |
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