Graphene-CNT nanohybrid aptasensor for label free detection of cardiac biomarker myoglobin

Kumar, Vinod ; Shorie, Munish ; Ganguli, Ashok K. ; Sabherwal, Priyanka (2015) Graphene-CNT nanohybrid aptasensor for label free detection of cardiac biomarker myoglobin Biosensors and Bioelectronics, 72 . pp. 56-60. ISSN 0956-5663

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.bios.2015.04.089

Abstract

We report a label free electrochemical detection of cardiac bio-marker myoglobin (Mb) on aptamer functionalized rGO/CNT nanostructured electrodes by measuring its direct electron transfer (DET). Configured as a highly responsive aptasensor, the newly developed biosensing platform exhibits synergistic effect of the nano-hybrid functional construct by combining good electrical properties and the facile chemical functionality of nanohybrid for the compatible bio-interface development. The specific anti-Mb aptamer was generated by five iterative SELEX (Systematic evolution of ligands by exponential enrichment) rounds, showing high senstivity (KD ~65 pM). The aptamer functionalized rGO/CNT nanostructured electrodes demonstrated a significant increase in signal response with a detection limit of ~0.34 ng/mL in the dynamic response range between 1 ng/mL and 4 µg/mL for Mb. The newly developed DET assay format presents a promising candidate in point-of-care diagnosis for routine screening of Mb in patient's samples.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Cardiac Marker; Myoglobin; Aptamer; rGO/CNT Nanohybrid; Direct Electron Transfer
ID Code:111621
Deposited On:26 Sep 2017 13:11
Last Modified:26 Sep 2017 13:11

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