Utility of saliva and hair follicles in donor selection for hematopoietic stem cell transplantation and chimerism monitoring

Kaur, Gurvinder ; Kumar, Neeraj ; Nandakumar, Ramya ; Rapthap, Chowphi C. ; Sharma, Gaurav ; Neolia, Shekhar ; Kumra, Heena ; Mahalwar, Prateek ; Garg, Abhinav ; Kumar, Sunil ; Kaur, Jasmeet ; Hakim, Mrinali ; Kumar, Lalit ; Mehra, Narinder K. (2012) Utility of saliva and hair follicles in donor selection for hematopoietic stem cell transplantation and chimerism monitoring Chimerism, 3 (1). pp. 9-17. ISSN 1938-1956

Full text not available from this repository.

Official URL: http://www.tandfonline.com/doi/abs/10.4161/chim.19...

Related URL: http://dx.doi.org/10.4161/chim.19395

Abstract

Selection of an HLA identical donor is a critical pre-requisite for successful hematopoietic stem cell transplantation (HSCT). Most transplant centers utilize blood as the most common source of DNA for HLA testing. However, obtaining blood through phlebotomy is often challenging in patients with conditions like severe leucopenia or hemophilia, pediatric and elderly patients. We have used a simple in-house protocol and shown that HLA genotypes obtained on DNA extracted from saliva or hair are concordant with blood and hence can be used for selection of donors for HSCT or organ transplantation. Similarly, for post-HSCT chimerism monitoring, non-availability of pre-transplant DNA samples poses a major limitation of reference STR fingerprints. This study shows that DNA obtained post-HSCT from hair follicles can be used to generate pre-transplant patient specific fingerprints while the STR profiles obtained in saliva samples cannot as these display a mixed state of chimerism.

Item Type:Article
Source:Copyright of this article belongs to Taylor & Francis Group.
Keywords:Saliva; Transplantation; Chimerism; HLA; DNA; Hair
ID Code:111081
Deposited On:25 Sep 2017 12:42
Last Modified:25 Sep 2017 12:42

Repository Staff Only: item control page