Biophysical characterization of Vitrio El Tor typing phage e5

Basu, Rina ; Ghosh, A. N. ; Dasgupta, S. ; Ghosh, Amit (1993) Biophysical characterization of Vitrio El Tor typing phage e5 FEMS Microbiology Letters, 106 (1). pp. 9-15. ISSN 0378-1097

Full text not available from this repository.

Official URL:

Related URL:


Vibrio cholerae typing phage e5, which can lyse only the El Tor strains of V. cholerae, was characterized. The phage had a polyhedral head 51 nm in diameter and a short tail 13 nm in length. It contained 13 structural polypeptides, with the molecular mass of the major component being 50 kDa. Phage chromosome comprised a 38.5-kb linear double-stranded DNA molecule with unique termini, as determined by restriction fragment analysis and electron microscopy, and had a G+C content of 35.5%. A physical map was constructed with the restriction endonucleases HaeII and HpaII. Adsorption of the phage to its host followed a biphasic kinetics and its intracellular growth was characterized by a latent period of 15 min and a burst size of 100 particles per infected cell. The phage was found to be moderately thermotolerant.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Microbiological Societies.
Keywords:Vibrio cholerae; Typing Phage; Restriction Map
ID Code:11084
Deposited On:09 Nov 2010 04:09
Last Modified:11 Feb 2011 11:46

Repository Staff Only: item control page