Construction of a recombinant live oral vaccine from a non-toxigenic strain of Vibrio cholerae O1 serotype inaba biotype El Tor and assessment of its reactogenicity and immunogenicity in the rabbit model

Thungapathra, M. ; Sharma, C. ; Gupta, N. ; Ghosh, R. K. ; Mukhopadhyay, A. ; Koley, H. ; Nair, G. Balakrish ; Ghosh, Amit (1999) Construction of a recombinant live oral vaccine from a non-toxigenic strain of Vibrio cholerae O1 serotype inaba biotype El Tor and assessment of its reactogenicity and immunogenicity in the rabbit model Immunology Letters, 68 (2-3). pp. 219-227. ISSN 0165-2478

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S01652...

Related URL: http://dx.doi.org/10.1016/S0165-2478(99)00076-0

Abstract

The disease cholera is an important cause of mortality in many developing countries. Though it can be controlled through improved sanitation, this goal is not easily attainable in many countries. Development of an efficacious vaccine offers the best immediate solution. A new oral candidate vaccine has been constructed from a non-toxigenic strain of Vibrio cholerae El Tor, Inaba, which is not only devoid of the cholera toxin (CT) virulence cassette but also is completely non-reactogenic in rabbit ileal loop assay. The strain, however, had toxR and tcpA genes. Through a series of manipulations, the ctxB gene of V. cholerae, responsible for the production of the 'B' subunit of the cholera toxin (CTB) was introduced into the cryptic hemolysin locus of the strain. The resulting strain, named vaccine attempt 1.3 (VA1.3), was found to be able to produce copious amounts of CTB. In the RITARD model this strain was found to be non-reactogenic and provided full protection against the challenge doses of both V. cholerae O1, classical and El Tor. In the immunized rabbit it invoked significant levels of anti-bacterial and anti-toxin immunity.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Cholera Toxin; Non-toxinogenic Strain; Oral Vaccine
ID Code:11081
Deposited On:09 Nov 2010 04:09
Last Modified:19 Jun 2012 14:13

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