A23187-Channel behaviour: fluorescence study

Jyothi, G. ; Surolia, A. ; Easwaran, K. R. K. (1994) A23187-Channel behaviour: fluorescence study Journal of Biosciences, 19 (3). pp. 277-282. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/19/277-282.pdf

Related URL: http://dx.doi.org/10.1007/BF02716816

Abstract

Pyranine entrapped soylipid liposomes have been used as a model system to study the proton transport across membrane in the presence of A23187, a carboxylic ionophore specific for electroneutral exchange of divalent cations. An apparent rate constant (kapp) for transport of protons has been determined from the rate of change of fluorescence intensity of pyranine by stopped flow rapid kinetics in the presence of proton gradient The variation of kapp has been studied as a function of ionophore concentration and the results have been compared with gramicidin-a well known channel former under the similar experimental conditions. The rates thus obtained showed that A23187 is not only a simple carrier but also shows channel behaviour at high concentration of ionophore.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:A23187-calcium Ionophore; Proton Transport; Stopped Flow Kinetics
ID Code:11044
Deposited On:09 Nov 2010 04:13
Last Modified:16 May 2016 20:33

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