A comprehensive model to predict mitotic division in budding yeasts

Sutradhar, Sabyasachi ; Yadav, Vikas ; Sridhar, Shreyas ; Sreekumar, Lakshmi ; Bhattacharyya, Dibyendu ; Ghosh, Santanu Kuma ; Paul, Raja ; Sanyal, Kaustuv (2015) A comprehensive model to predict mitotic division in budding yeasts Molecular Biology of the Cell, 26 (22). pp. 3954-3965. ISSN 1059-1524

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Official URL: http://www.molbiolcell.org/content/26/22/3954

Related URL: http://dx.doi.org/10.1091/mbc.E15-04-0236


High-fidelity chromosome segregation during cell division depends on a series of concerted interdependent interactions. Using a systems biology approach, we built a robust minimal computational model to comprehend mitotic events in dividing budding yeasts of two major phyla: Ascomycota and Basidiomycota. This model accurately reproduces experimental observations related to spindle alignment, nuclear migration, and microtubule (MT) dynamics during cell division in these yeasts. The model converges to the conclusion that biased nucleation of cytoplasmic microtubules (cMTs) is essential for directional nuclear migration. Two distinct pathways, based on the population of cMTs and cortical dyneins, differentiate nuclear migration and spindle orientation in these two phyla. In addition, the model accurately predicts the contribution of specific classes of MTs in chromosome segregation. Thus we present a model that offers a wider applicability to simulate the effects of perturbation of an event on the concerted process of the mitotic cell division.

Item Type:Article
Source:Copyright of this article belongs to American Society for Cell Biology.
ID Code:109846
Deposited On:25 Oct 2017 13:09
Last Modified:25 Oct 2017 13:09

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