Shetty, Ronak ; Inamdar, Maneesha S. (2016) Generation of a constitutively expressing tetracycline repressor (TetR) human embryonic stem cell line BJNhem20-TetR Stem Cell Research, 16 (2). pp. 271-273. ISSN 1873-5061
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.scr.2015.12.043
Abstract
Human embryonic stem cell line BJNhem20-TetR was generated using non-viral method. The construct pCAG-TetRnls was transfected using microporation procedure. BJNhem20-TetR can subsequently be transfected with any vector harbouring a TetO (Tet operator) sequence to generate doxycycline based inducible line. For example, in human embryonic stem cells, the pSuperior based TetO system has been transfected into a TetR containing line to generate OCT4 knockdown cell line (Zafarana et al., 2009). Thus BJNhem20-TetR can be used as a tool to perturb gene expression in human embryonic stem cells.
| Item Type: | Article |
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| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 109190 |
| Deposited On: | 01 Feb 2018 04:55 |
| Last Modified: | 01 Feb 2018 04:55 |
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