Identification and characterization of the tyrosine protein kinases of rat spleen

Tokuda, Masaaki ; Khanna, Navin C. ; Arora, Akhil K. ; Waisman, David M. (1986) Identification and characterization of the tyrosine protein kinases of rat spleen Biochemical and Biophysical Research Communications, 139 (3). pp. 910-917. ISSN 0006-291X

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/S0006-291X(86)80264-9

Abstract

High levels of tyrosine protein kinase have been recently detected in the membranes of rat spleen. In the present report the tyrosine protein kinase activity of the 30,000 x g pellet of rat spleen has been solubilized and partially purified by ion exchange and gel permeation chromatography. Two peaks of tyrosine protein kinase of Mr 35,000 (TPK-I) and Mr 40,000 (TPK-II) have been resolved. These kinases were free of the EGF receptor and insulin receptor tyrosine protein kinases. Although TPK-I and TPK-II phosphorylated angiotensin II, casein, histone, tubulin, phosphorylase b and p36 they differed from each other in preference for the substrates. Both tyrosine protein kinases did not phosphorylate anti-pp60v-src IgG.

Item Type:Article
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ID Code:109133
Deposited On:09 Mar 2018 12:14
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