Induction of neutralizing antibodies specific to dengue virus serotypes 2 and 4 by a bivalent antigen composed of linked envelope domains III of these two serotypes

Khanam, Saima ; Etemad, Behzad ; Khanna, Navin ; Swaminathan, Sathyamangalam (2006) Induction of neutralizing antibodies specific to dengue virus serotypes 2 and 4 by a bivalent antigen composed of linked envelope domains III of these two serotypes The American Journal of Tropical Medicine and Hygiene, 74 (2). pp. 266-277. ISSN 0002-9637

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Official URL: http://www.ajtmh.org/content/journals/10.4269/ajtm...

Related URL: http://dx.doi.org/10.4269/ajtmh.2006.74.266

Abstract

There is no vaccine to prevent dengue fever, a mosquito-borne viral disease, caused by four serotypes of dengue viruses. In this study, which has been prompted by the emergence of dengue virus envelope domain III as a promising sub-unit vaccine candidate, we have examined the possibility of developing a chimeric bivalent antigen with the potential to elicit neutralizing antibodies against two serotypes simultaneously. We created a chimeric dengue antigen by splicing envelope domain IIIs of serotypes 2 and 4. It was expressed in Escherichia coli and purified to near homogeneity. This protein retains the antigenic identitities of both its precursors. It elicited antibodies that could efficiently block host cell binding of both serotypes 2 and 4 of dengue virus and neutralize their infectivity (neutralizing antibody titers approximately 1:40 and ∼1:80 for dengue virus serotypes 2 and 4, respectively). This work could be a forerunner to the development of a single envelope domain III-based tetravalent antigen.

Item Type:Article
Source:Copyright of this article belongs to American Society of Tropical Medicine and Hygiene.
ID Code:109091
Deposited On:09 Mar 2018 12:12
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