Dengue-specific subviral nanoparticles: design, creation and characterization

Khetarpal, Niyati ; Poddar, Ankur ; Nemani, Satish K. ; Dhar, Nisha ; Patil, Aravind ; Negi, Priyanka ; Perween, Ashiya ; Viswanathan, Ramaswamy ; Lunsdorf, Heinrich ; Tyagi, Poornima ; Raut, Rajendra ; Arora, Upasana ; Jain, Swatantra K. ; Rinas, Ursula ; Swaminathan, Sathyamangalam ; Khanna, Navin (2013) Dengue-specific subviral nanoparticles: design, creation and characterization Journal of Nanobiotechnology, 11 (1). Article ID 15. ISSN 1477-3155

[img]
Preview
PDF
721kB

Official URL: https://jnanobiotechnology.biomedcentral.com/artic...

Related URL: http://dx.doi.org/10.1186/1477-3155-11-15

Abstract

Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like Particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus Envelope protein Domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate. Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally. Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence. Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.

Item Type:Article
Source:Copyright of this article belongs to BioMed Central.
Keywords:Dengue Envelope Domain III; Hepatitis B Surface Antigen; Virus-like Particle; Bionanoparticles; Pichia Pastoris
ID Code:109029
Deposited On:09 Mar 2018 12:10
Last Modified:09 Mar 2018 12:10

Repository Staff Only: item control page