Excipients activate peroxidases in specific but not in non-specific reactions in organic solvents

Yuchun, Xie ; Das, Prasanta Kumar ; Klibanov, Alexander M. (2001) Excipients activate peroxidases in specific but not in non-specific reactions in organic solvents Biotechnology Letters, 23 (18). pp. 1451-1454. ISSN 0141-5492

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Official URL: https://link.springer.com/article/10.1023/A:101164...

Related URL: http://dx.doi.org/10.1023/A:1011641622960

Abstract

Co-lyophilization with ligands and lyoprotectants markedly activated horseradish and soybean peroxidases in the oxidation of guaiacol in organic solvents (Dai and Klibanov (1999) Proc. Natl. Acad. Sci. USA96: 9475–9478). However, such excipients gave little or no activation of these enzymes in non-specific reactions, sulfoxidation of thioanisole and epoxidation of styrene, presumably because they do not require a precise fine tuning of the enzyme active site.

Item Type:Article
Source:Copyright of this article belongs to Springer Verlag.
Keywords:Expoxidations; Lyophilization; Nonaqueous Biocatalysis; Peroxidase; Sulfoxidations
ID Code:108779
Deposited On:01 Feb 2018 11:24
Last Modified:01 Feb 2018 11:24

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