Unsaturation at the surfactant head: Influence on the activity of lipase and horseradish peroxidase in reverse micelles

Debnath, Sisir ; Das, Dibyendu ; Das, Prasanta Kumar (2007) Unsaturation at the surfactant head: Influence on the activity of lipase and horseradish peroxidase in reverse micelles Biochemical and Biophysical Research Communications, 356 (1). pp. 163-168. ISSN 0006-291X

Full text not available from this repository.

Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.bbrc.2007.02.132

Abstract

Influence of unsaturation present at the surfactant head on the activity of interfacially located enzyme, lipase, and horseradish peroxidase (HRP) is investigated in cationic reverse micelles of a series of surfactants having unsaturated (allyl and pyridinium moieties) as well as analogous saturated (n-propyl and piperidinium moieties) polar head. Lipase activity increases with n-propyl (saturated) substitution as the increase in the headgroup area (Amin) presumably provides greater space for the enzyme to attain flexible conformation and increases the local concentrations of enzyme and substrate at the interface. In contrast, activity of lipase decreases with increasing number of allyl (unsaturated) substitution though Amin gradually increased. Similar trend in deactivation was observed when unsaturation is present in cyclic ring (pyridine) at the surfactant head in comparison to the saturated analogue, piperidine. Circular dichroism (CD) spectra of lipase in reverse micelles indicate that ellipticity in the far-UV region increases with increasing unsaturation. Thus, lipase probably loses its α-helix content and thereby its activity. Inhibition of biocatalyst with increasing unsaturation at the polar head of surfactant is also observed in case of HRP, an oxidoreductase enzyme.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Amphiphiles; Enzymes; Inhibition; Reverse Micelles; Surfactant Head; Unsaturation
ID Code:108739
Deposited On:01 Feb 2018 11:19
Last Modified:01 Feb 2018 11:19

Repository Staff Only: item control page