Datta, S. K. ; Wenzel, G. (1987) Isolated microspore derived plant formation via embryogenesis in Triticum aestivum L. Plant Science, 48 (1). pp. 49-54. ISSN 0168-9452
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0168-9452(87)90069-0
Abstract
Embryogenesis in isolated microspores of wheat (Triticum aestivum L.) leading to plant regeneration has been established on modified liquid N6 medium (supplemented with 2,4-D, casein hydrolysate and Ficoll). Globular embryoids which were obtained after 6–8 weeks of culture of competent embryogenic microspores produced perfect embryoids when transferred to regeneration medium. Embryoids were differentiated to plants on other modified N6 agar medium (0.75% w/v agar, 20 g/l sucrose, 1 g/l myo-inositol, 8.8 μM 6-benzylaminopurine (BAP), 11.4 μM indoleacetic acid (IAA), 160 mg/l glutamine, 10 mg/l proline). Responses of microspores in regeneration and embryoid differentiation varied depending on the constituents of the media and genotypes used.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Triticum aestivum; Microspore Culture; Embryogenesis; Haploids |
| ID Code: | 108558 |
| Deposited On: | 01 Feb 2018 11:10 |
| Last Modified: | 01 Feb 2018 11:10 |
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