Artificial seeds in barley: encapsulation of microspore-derived embryos

Datta, S. K. ; Potrykus, I. (1989) Artificial seeds in barley: encapsulation of microspore-derived embryos Theoretical And Applied Genetics, 77 (6). pp. 820-824. ISSN 0040-5752

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An in vitro culture system has been developed for barley (Hordeum vulgare), which yields high frequencies of high quality microspore-derived embryos without an intervening callus phase. The embryos are very similar to zygotic embryos with regard to their morphology and germination capacity. These embryos were encapsulated in sodium alginate to produce individual beads containing one embryo each. In accordance with the literature, these beads are denoted “artificial seeds”. The artificial seeds germinated well and with a root system superior to that of non-encapsulated embryos. The artificial seeds also maintained their germination capacity for at least 6 months, whereas non-encapsulated embryos did not survive more than 2 weeks in storage. Artificial seeds, thus, probably provide a simple and universal delivery system of in vitro plantlets to greenhouse or field.

Item Type:Article
Source:Copyright of this article belongs to Springer Verlag.
Keywords:Artificial Seeds; Direct Microspore Embryogenesis; Storage; Hordeum vulgare; Haploidy
ID Code:108539
Deposited On:01 Feb 2018 11:10
Last Modified:01 Feb 2018 11:10

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