Genetic stability developed for β-carotene synthesis in BR29 rice line using dihaploid homozygosity

Datta, Karabi ; Sahoo, Gayetri ; Krishnan, Sellappan ; Ganguly, Moumita ; Datta, Swapan K. (2014) Genetic stability developed for β-carotene synthesis in BR29 rice line using dihaploid homozygosity PLoS One, 9 (6). Article ID e100212-8 pages. ISSN 1932-6203

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Official URL: http://journals.plos.org/plosone/article?id=10.137...

Related URL: http://dx.doi.org/10.1371/journal.pone.0100212

Abstract

Obtaining transgenic crop lines with stable levels of carotenoids is highly desirable. We addressed this issue by employing the anther culture technique to develop dihaploid lines containing genes involved in β-carotene metabolism. First, we used Agrobacterium- mediated transformation to develop primary transgenic plants containing the β-carotene biosynthetic genes, phytoene synthase (psy) and phytoene desaturase (crtl), which were engineered for expression and accumulation in the endosperm. Transgenic plants were recovered by selecting for the expression of the phosphomannose isomerase (pmi) gene. Dihaploid plants in addition to haploid and tetraploid plant were generated from anther cultures of these primary transgenic plants. In addition to anatomical features of stomata, pollen of different ploidy-plants, molecular analyses confirmed the stable integration of the genes in the anther culture-derived dihaploid plants, and the yellow color of the polished seeds indicated the accumulation of carotenoids in the endosperm. High performance liquid chromatography (HPLC) analysis of the carotenoid extract further confirmed the levels of β–carotene accumulation in the endosperms of the transgenic dihaploid rice seeds.

Item Type:Article
Source:Copyright of this article belongs to Public Library of Science.
ID Code:108537
Deposited On:01 Feb 2018 11:10
Last Modified:01 Feb 2018 11:10

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