Molecular characterization of marker-free transgenic lines of indica rice that accumulate carotenoids in seed endosperm

Parkhi, V. ; Rai, M. ; Tan, J. ; Oliva, N. ; Rehana, S. ; Bandyopadhyay, A. ; Torrizo, L. ; Ghole, V. ; Datta, K. ; Datta, S. K. (2005) Molecular characterization of marker-free transgenic lines of indica rice that accumulate carotenoids in seed endosperm Molecular Genetics and Genomics, 274 (4). pp. 325-336. ISSN 1617-4615

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Official URL: https://link.springer.com/article/10.1007%2Fs00438...

Related URL: http://dx.doi.org/10.1007/s00438-005-0030-7

Abstract

A single Agrobacterium strain harbouring two binary plasmids was successfully used for the first time to develop a marker-free transgenic rice of improved nutritional value. Sixty-eight T0 co-transformants were obtained in three indica rice cultivars—two popular high-yielding Bangladeshi varieties (BR28 and BR29), and one high-iron rice cultivar (IR68144). Marker-free lines were obtained from 14 out of 24 selected co-transformants screened in the T1 generation. The accumulation of total carotenoids in polished T2 rice seeds of the primary transgenic VPBR29-17-37 reached levels of up to 3.0 μg/g, with the level of β-carotene reaching 1.8 μg/g. In the cultivars BR28 and IR68144, total carotenoid levels in the transformants reached 2.0 μg/g of polished rice seeds. The levels of lutein and other carotenoids in the seeds were also significantly enhanced. T1 plants obtained from primary transgenics with simple gene-integration patterns tended to have a lower carotenoid content than the original parental lines. This study describes the development of marker-free transgenic rice lines containing high levels of carotenoids, and addresses the relationship between the rearrangement of transgenes and the presence of metabolic end products in transgenic rice.

Item Type:Article
Source:Copyright of this article belongs to Springer Verlag.
Keywords:Agrobacterium Tumefaciens; Co-Transformation; High-Iron Rice; β-Carotene; HPLC
ID Code:108533
Deposited On:01 Feb 2018 11:10
Last Modified:01 Feb 2018 11:10

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