Engineering preferences of hairpin PNA binding to complementary DNA: effect of N7G in aeg/aep PNA backbone

Kumar, V. A. ; D'Costa, M. ; Ganesh, K. N. (2001) Engineering preferences of hairpin PNA binding to complementary DNA: effect of N7G in aeg/aep PNA backbone Nucleosides, Nucleotides and Nucleic Acids, 20 (4-7). pp. 1187-1191. ISSN 1525-7770

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Related URL: http://dx.doi.org/10.1081/NCN-100002516

Abstract

AegPNA and aepPNA monomeric units bearing the N7-guanine nucleobase as a substitute for C+ have been demonstrated to bind to a GC base-pair of a duplex in a pH-independent manner when placed in the third strand. The aepPNA backbone exerts a preference for binding in the antiparallel Hoogsteen mode over the parallel Hoogsteen mode.

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