Rajendrakumar, G. V. ; Sivakamisundari, N. ; Ganesh, K. N. (1997) Rapid chemical synthesis of d(CACGTG) in milligram amounts by solution-phase phosphotriester chemistry Proceedings of the Indian Academy of Sciences - Chemical Sciences, 99 (5-6). pp. 341-350. ISSN 0253-4134
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Official URL: http://www.ias.ac.in/j_archive/chemsci/99/3/341-35...
Related URL: http://dx.doi.org/10.1007/BF02880457
Abstract
This paper reports an optimised method for chemical synthesis of short oligodeoxyribonucleotides in milligram amounts by solution-phase phosphotriester chemistry. The procedure is a slight modification of one reported earlier in the literature. Detailed experimental procedures for condensation, deprotection and purification are described. The efficiency of the method is demonstrated by the synthesis of a hexamer, d(CACGTG), in good yields and high purity. The homogeneity of the product and the sequence composition are shown by 270 MHz1H NMR spectrum.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Indian Academy of Sciences. |
| Keywords: | Oligodeoxyribonucleotide Synthesis; Chemical Synthesis of d(CACGTG); 1H NMR of Oligonucleotides |
| ID Code: | 10852 |
| Deposited On: | 09 Nov 2010 04:41 |
| Last Modified: | 16 May 2016 20:23 |
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