Down-regulation of lipoxygenase gene reduces degradation of carotenoids of golden rice during storage

Gayen, Dipak ; Ali, Nusrat ; Sarkar, Sailendra Nath ; Datta, Swapan K. ; Datta, Karabi (2015) Down-regulation of lipoxygenase gene reduces degradation of carotenoids of golden rice during storage Planta, 242 (1). pp. 353-363. ISSN 0032-0935

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Bio-fortified provitamin A-enriched rice line (golden rice) expressing higher amounts of β-carotene in the rice endosperm provides vitamin A for human health. However, it is already reported that degradation of carotenoids during storage is a major problem. The gene responsible for degradation of carotenoids during storage has remained largely unexplored till now. In our previous study, it has been shown that r9-LOX1 gene is responsible for rice seed quality deterioration. In the present study, we attempted to investigate if r9-LOX1 gene has any role in degradation of carotenoids in rice seeds during storage. To establish our hypothesis, the endogenous lipoxygenase (LOX) activity of high-carotenoid golden indica rice seed was silenced by RNAi technology using aleurone layer and embryo-specific Oleosin-18 promoter. To check the storage stability, LOX enzyme down-regulated high-carotenoid T3 transgenic rice seeds were subjected to artificial aging treatment. The results obtained from biochemical assays (MDA, ROS) also indicated that after artificial aging, the deterioration of LOX-RNAi lines was considerably lower compared to β-carotene-enriched transgenic rice which had higher LOX activity in comparison to LOX-RNAi lines. Furthermore, it was also observed by HPLC analysis that down-regulation of LOX gene activity decreases co-oxidation of β-carotene in LOX-RNAi golden rice seeds as compared to the β-carotene-enriched transgenic rice, after artificial aging treatment. Therefore, our study substantially establishes and verifies that LOX is a key enzyme for catalyzing co-oxidation of β-carotene and has a significant role in deterioration of β-carotene levels in the carotenoid-enriched golden rice.

Item Type:Article
Source:Copyright of this article belongs to Springer Verlag.
Keywords:RNAi; β-Carotene; Provitamin A; Lipoxygenase; Post-Harvest Deterioration; Artificial Aging
ID Code:108500
Deposited On:01 Feb 2018 11:08
Last Modified:01 Feb 2018 11:08

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