Datta, Swapan K. ; Datta, Karabi ; Potrykus, Ingo (1990) Fertile Indica rice plants regenerated from protoplasts isolated from microspore derived cell suspensions Plant Cell Reports, 9 (5). pp. 253-256. ISSN 0721-7714
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Official URL: https://link.springer.com/article/10.1007/BF002322...
Related URL: http://dx.doi.org/10.1007/BF00232295
Abstract
Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is described. Regenerating protoplasts could readily be isolated from 5–12 months old cell suspensions showing visible colony formation in the range of 180–1050 colonies/106 protoplasts after about one month in culture. More than 100 independent green plantlets were regenerated via secondary embryogenesis from ca 20×106 protoplasts. Out of 32 plants grown to maturity under greenhouse conditions 24 were fertile.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer Verlag. |
| ID Code: | 108492 |
| Deposited On: | 01 Feb 2018 11:08 |
| Last Modified: | 01 Feb 2018 11:08 |
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