Jadhav, Vasant R. ; Barawkar, Dinesh A. ; Natu, Arvind A. ; Ganesh, Krishna N. (1997) 5-amido-(carboxyfluorescein)-2'-dU-oligonucloetides: novel primers for fluorescent detection of PCR amplified DNA Nucleosides, Nucleotides and Nucleic Acids, 16 (1-2). pp. 107-114. ISSN 1525-7770
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Official URL: http://www.informaworld.com/smpp/content~db=all~co...
Related URL: http://dx.doi.org/10.1080/07328319708002526
Abstract
The synthesis of oligonucleotides containing a novel nucleobase fluorescent derivative 5-NH2-(carboxyfluorescein)-2'-deoxyuridine, in which the chromophore fluorescein is directly linked to nucleobase of 2'dU at C-5 via an amido function is described. The fluorescent oligonucleotides have been used as primers for PCR amplification of Human β-globin gene fragment. Oligonucleotides containing multiple insertions of such fluorophores have potential applications in molecular biotechnology.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Taylor and Francis Ltd. |
| ID Code: | 10844 |
| Deposited On: | 09 Nov 2010 04:42 |
| Last Modified: | 31 May 2011 09:51 |
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