Enhanced iron and zinc accumulation in transgenic rice with the ferritin gene

Vasconcelos, Marta ; Datta, Karabi ; Oliva, Norman ; Khalekuzzaman, Mohammad ; Torrizo, Lina ; Krishnan, Sellapan ; Oliveira, Margarida ; Goto, Fumyuki ; Datta, Swapan K. (2003) Enhanced iron and zinc accumulation in transgenic rice with the ferritin gene Plant Science, 164 (3). pp. 371-378. ISSN 0168-9452

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/S0168-9452(02)00421-1


In this report, we show that the expression of the soybean ferritin gene, driven by the endosperm-specific glutelin promoter, leads to higher iron and zinc levels in transgenic indica rice grains. Brown rice is rarely consumed, and polishing of the rice grain brings considerable loss of micronutrients by removing its outer layers. No data until now have shown that after commercial milling the micronutrient concentration remains higher than that of the control. In our experiment, expression of the soybean ferritin gene under the control of the glutelin promoter in rice has proven to be effective in enhancing grain nutritional levels, not only in brown grains but also in polished grains. Besides determining the iron levels in transgenic rice grains, we also checked for zinc concentration, and it was found to be higher in transgenic seeds than in the control. Moreover, we introduced this gene in an elite indica rice line that has highly desirable agronomic and field-performance traits. Prussian blue staining reaction clearly revealed the presence of iron in the endosperm cells of transgenic rice grains, and immunolocalization revealed the presence of the expression gene in the endosperm of the transgenic material.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Iron Accumulation; Milling; Genetic Engineering; Nutrition; Elite indica Rice
ID Code:108415
Deposited On:01 Feb 2018 11:08
Last Modified:01 Feb 2018 11:08

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