Minganti, Carlo ; Ganesh, K. N. ; Sproat, Brian S. ; Gait, Michael J. (1985) Comparison of controlled pore glass and Kieselguhr-polydimethylacrylamide composite as supports for solid-phase synthesis of 23-residue oligodeoxyribonucleotides in milligram amounts Analytical Biochemistry, 147 (1). pp. 63-74. ISSN 0003-2697
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000326...
Related URL: http://dx.doi.org/10.1016/0003-2697(85)90009-0
Abstract
Two 23-residue oligodeoxyribonucleotides, corresponding to both strands of a DNA duplex at the OR3 site of bacteriophage λ, have been synthesized in good yields and in milligram quantities by a solid-phase phosphotriester method using two different supports, Kieselguhr-polydimethylacrylamide composite and controlled pore glass. Rapid purification was possible using high-performance liquid chromatography on radial compression ion-exchange columns. The results and utility of the two supports are compared.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Oligodeoxyribonucleotide Synthesis; Solid-phase; Ion-exchange HPLC; Radial Compression; Solid Support; λOR3 Recognition Site |
| ID Code: | 10831 |
| Deposited On: | 09 Nov 2010 04:45 |
| Last Modified: | 31 May 2011 10:07 |
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