Genetically engineered fertile indica-rice recovered from protoplasts

Datta, Swapan K. ; Peterhans, Alex ; Datta, Karabi ; Potrykus, Ingo (1990) Genetically engineered fertile indica-rice recovered from protoplasts Nature Biotechnology, 8 (8). pp. 736-740. ISSN 1087-0156

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We have established an efficient protocol for plant regeneration from haploid Indica-type rice protoplasts. Incubation of these protoplasts with the selectable hygromycin phosphotransferase (hph) gene expressed under control of the 35S promoter of cauliflower mosaic virus (CaMV) and polyethyleneglycol (PEG), and subsequent culture in the presence of hygromycin B, led to the recovery of numerous resistant clones from which 77 plants were regenerated. Data from Southern analysis and enzyme assays proved that the transgene was stably integrated into the host genome and expressed, and that it was inherited in offspring.

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