Allosteric regulation of tRNA import: interactions between tRNA domains at the inner membrane of Leishmania mitochondria

Goswami, Srikanta ; Chatterjee, Saibal ; Bhattacharyya, Suvendra Nath ; Basu, Sudarshana ; Adhya, Samit (2003) Allosteric regulation of tRNA import: interactions between tRNA domains at the inner membrane of Leishmania mitochondria Nucleic Acids Research, 31 (19). pp. 5552-5559. ISSN 1362-4962

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Official URL: https://academic.oup.com/nar/article/31/19/5552/10...

Related URL: http://dx.doi.org/10.1093/nar/gkg773

Abstract

Import of nucleus‐encoded tRNAs into the mitochondria of the kinetoplastid protozoon Leishmania involves recognition of specific import signals by the membrane‐bound import machinery. Multiple signals on different tRNA domains may be present, and further, importable RNAs interact positively (Type I) or negatively (Type II) with one another at the inner membrane in vitro. By co‐transfection assays, it is shown here that tRNATyr (Type I) transiently stimulates the rate of entry of tRNAIle (Type II) into Leishmania mitochondria in transfected cells, and conversely, is inhibited by tRNAIle. Truncation and mutagenesis experiments led to the co‐localization of the effector and import activities of tRNATyr to the D domain and those of tRNAIle to the variable region–T domain (V‐T region), indicating that both activities originate from a single RNA–receptor interaction. A third tRNA, human tRNALys, is imported into Leishmania mitochondria in vitro as well as in vivo. This tRNA has Type I and Type II motifs in the D domain and the V‐T region, respectively, and shows both Type I and Type II effector activities. Such dual‐type tRNAs may interact simultaneously with the Type I and Type II binding sites of the inner membrane import machinery.

Item Type:Article
Source:Copyright of this article belongs to Oxford University Press.
ID Code:108158
Deposited On:01 Feb 2018 11:12
Last Modified:01 Feb 2018 11:12

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