Kamal, Ahmed ; Reddy, K. Srinivasa ; Khan, M. Naseer A. ; Shetti, Rajesh V. C. R. N. C. ; Ramaiah, M. Janaki ; Pushpavalli, S. N. C. V. L. ; Srinivas, Chatla ; Pal-Bhadra, Manika ; Chourasia, Mukesh ; Sastry, G. Narahari ; Juvekar, Aarti ; Zingde, Surekha ; Barkume, Madan (2010) Synthesis, DNA-binding ability and anticancer activity of benzothiazole/benzoxazole–pyrrolo[2,1-c][1,4]benzodiazepine conjugates Bioorganic & Medicinal Chemistry, 18 (13). pp. 4747-4761. ISSN 0968-0896
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.bmc.2010.05.007
Abstract
A series of benzothiazole and benzoxazole linked pyrrolobenzodiazepine conjugates attached through different alkane or alkylamide spacers was prepared. Their anticancer activity, DNA thermal denaturation studies, restriction endonuclease digestion assay and flow cytometric analysis in human melanoma cell line (A375) were investigated. One of the compounds of the series 17d showed significant anticancer activity with promising DNA-binding ability and apoptosis caused G0/G1 phase arrest at sub-micromolar concentrations. To ascertain the binding mode and understand the structural requirement of DNA binding interaction, molecular docking studies using gold program and more rigorous 2 ns molecular dynamic simulations using Molecular Mechanics-Poisson–Boltzman Surface Area (MM-PBSA) approach including the explicit solvent were carried out. Further, the compound 17d was evaluated for in vivo efficacy studies in human colon cancer HT29 xenograft mice.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Pyrrolobenzodiazepine; Benzothiazole; Benzoxazole, DNA-Binding Affinity; Anticancer Activity; Molecular Modelling; In vivo Efficacy |
| ID Code: | 108099 |
| Deposited On: | 28 Jul 2017 05:51 |
| Last Modified: | 28 Jul 2017 05:51 |
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