EPHX1 gene polymorphisms in alcohol dependence and their distribution among the Indian populations

Bhaskar, L. V. K. S. ; Thangaraj, K. ; Patel, Minarbha ; Shah, Anish M. ; Gopal, K. ; Saikrishna, L. ; Tamang, Rakesh ; Singh, Lalji ; Rao, V. R. (2013) EPHX1 gene polymorphisms in alcohol dependence and their distribution among the Indian populations American Journal of Drug and Alcohol Abuse, 39 (1). pp. 16-22. ISSN 0095-2990

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Official URL: http://www.tandfonline.com/doi/abs/10.3109/0095299...

Related URL: http://dx.doi.org/10.3109/00952990.2011.643991


Background: The microsomal epoxide hydrolase is a phase II enzyme of the biotransformation. The human Epoxide Hydrolase 1 (EPHX1) gene lies in the chromosomal region 1q42.1 and exhibits polymorphism. Two Single Nucleotide Polymorphisms (SNPs) have been described in the coding region of the EPHX1 gene that produces two protein variants. Subjects and Methods: A total of 604 samples belonging to 13 Indian populations were included in this study. Based on the DSM-IV criteria, 184 individuals from Kota population were classified into alcoholism cases (100) and controls (84). Genotypes of Tyr113His and His139Arg polymorphisms in the EPHX1 gene were determined using PCR and sequencing. Associations were tested using Pearson’s χ2 test and haplotype analyses. Results: We found significant association between EPHX1gene Tyr113His polymorphism and alcoholism in the Kota population (T vs. C: OR = .615, 95% CI = .399–.949, p = .027; TT vs. CC + CT: OR = .536, 95% CI = .297–.969, p = .038). The very slow activity haplotype CA (113His–139His) was also found to be associated with alcohol dependence (p = .048). Analysis of additional populations demonstrated that the Tyr113His polymorphism significantly deviated from Hardy–Weinberg equilibrium in four populations but only one population deviated for the His139Arg locus. All populations shared the four possible two-site haplotypes. Linkage disequilibrium between these two loci was not significant in any of the population studied. Conclusion: EPHX1 gene polymorphisms and haplotypes are associated with an increased risk for alcoholism in the Kota population. This is the first report from India that will serve as a template for future investigations of the prevalence of EPHX1 alleles in association with various clinical entities.

Item Type:Article
Source:Copyright of this article belongs to Taylor & Francis.
Keywords:EPHX1; SNP; Allelic Variation; India
ID Code:107733
Deposited On:21 Jul 2017 12:00
Last Modified:21 Jul 2017 12:00

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