Fusarium solani: immunochemical characterization of allergens

Verma, Jyotsna ; Gangal, Sharad V. (1994) Fusarium solani: immunochemical characterization of allergens International Archives of Allergy and Immunology, 104 (2). pp. 175-183. ISSN 1018-2438

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Related URL: http://dx.doi.org/10.1159/000236727


Allergenic components of the fungus Fusarium solani were isolated using (NH4)2SO4 precipitation and ion-exchange column chromatography. The allergenicity of fractions was studied by enzyme-linked immunosorbent assay and radioallergosorbent test inhibition techniques. Proteins of culture filtrate (CF), mycelium (MY), and spore (SP) extracts of F. solani were characterized by isoelectrofocusing, sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and IgE-specific immunoblotting. CF antigen of F. solani contained more allergenic proteins than MY and SP, visible on immunoblot analysis using allergenic serum pool. A 65-kD protein component of CF was found to be a major allergen, as it was strongly visible on immunoblots of all 15 patient sera tested. Crossed immunoelectrophoresis and enzyme-linked immunosorbent assay inhibition using rabbit antibodies raised against F solani CF demonstrated shared antigenicity between CF, MY, and SP extracts. It was observed that F. solani is a significant allergen, and most of the allergens of MY and SP extracts were found in CF extract. Therefore, CF alone can be used in the preparation of a standard extract. However, few unique allergenic proteins were observed in MY as well as in SP extracts of F. solani. Hence, the use of combined CF, MY, and SP extracts of F. solani is recommended for diagnosis and immunotherapy.

Item Type:Article
Source:Copyright of this article belongs to Karger Publisher.
Keywords:Culture Filtrate; Fusarium solani Allergens; Standardization; Radioallergosorbent Test
ID Code:10682
Deposited On:04 Nov 2010 07:06
Last Modified:01 Jun 2011 11:47

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