Flagella as a novel alignment medium for the measurement of residual dipolar couplings in proteins

Singh, Himanshu ; Shukla, Manish ; Rao, Basuthkar J. ; Chary, Kandala V. R. (2013) Flagella as a novel alignment medium for the measurement of residual dipolar couplings in proteins Chemical Communications, 49 (97). pp. 11403-11405. ISSN 1359-7345

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Official URL: http://pubs.rsc.org/en/Content/ArticleLanding/2013...

Related URL: http://dx.doi.org/10.1039/C3CC46233A

Abstract

The two flexible rod-like flagella (∼500 nm in diameter and 5–15 μm long) of Chlamydomonas reinhardtii, a unicellular green alga, can weakly align molecules in an external magnetic field, thereby enabling the measurement of various residual dipolar couplings in solution NMR spectroscopy.

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