Purification and characterization of allergens from Xanthium strumarium pollen

Jaggi, Kuldeep S. ; Gangal, Sharad V. (1987) Purification and characterization of allergens from Xanthium strumarium pollen Molecular and Cellular Biochemistry, 78 (2). pp. 177-190. ISSN 0300-8177

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Official URL: http://www.springerlink.com/content/l51h8w70631713...

Related URL: http://dx.doi.org/10.1007/BF00229692


The allergenic components present in whole pollen extract of Xanthium strumarium were isolated by sequential ammonium sulphate precipitation, DEAE Sephadex A50 chromatography and gel filtration. The techniques of RAST inhibition and skin test were utilized to check the allergenicity of fractionated proteins revealing the presence of Xan Ib and Xan VIa as the important allergenic componenets. Xan Ib was found to be devoid of carbohydrate and had a molecular weight of 103 000 daltons. Xan VIa was a glycoprotein of molecular weight 17 000 daltons. The carbohydrate moiety of Xan Vla was found to be associated with allergenicity. The characteristic pattern of whole pollen extract on CIE and TLIEF showed 36 and 21 protein bands, respectively. The use of FPLC in isolation of partially purified allergens from Xanthium is discussed.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:Allergens; Xanthium Pollen
ID Code:10675
Deposited On:04 Nov 2010 07:05
Last Modified:01 Jun 2011 12:06

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