Singh, Himanshu ; Rao, B. J. ; Chary, Kandala V. R. (2014) 1H, 13C and 15N NMR assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii Biomolecular NMR Assignments, 8 (2). pp. 371-374. ISSN 1874 2718
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Official URL: http://link.springer.com/article/10.1007%2Fs12104-...
Related URL: http://dx.doi.org/10.1007/s12104-013-9520-4
Abstract
Almost complete sequence specific 1H, 13C and 15N resonance assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii are reported, as a prelude to its structural and functional characterization. Site-directed mutagenesis of uvi31+ was carried out using complementary mutation harbouring oligonucleotides for S55A mutant. The resulting mutant was sequenced and then S55A mutant of uvi31+ cDNA were expressed in E. coli, and purification were carried out from where the protein was purified to high homogeneity. The point mutation S55A in UVI31+ results in a significant enhancement in its DNA endonuclease activity as compared to its wild type protein.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer Verlag. |
| Keywords: | NMR; Resonance Assignments; UVI31+; S55A; Chlamydomonas reinhardtii |
| ID Code: | 106626 |
| Deposited On: | 15 Jun 2017 10:53 |
| Last Modified: | 15 Jun 2017 10:53 |
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