Immunochemical characterization of Cocos nucifera pollen

Jaggi, Kuldeep S. ; Arora, Naveen ; Niphadkar, Pramod V. ; Gangal, Sharad V. (1989) Immunochemical characterization of Cocos nucifera pollen Journal of Allergy and Clinical Immunology, 84 (3). pp. 378-385. ISSN 0091-6749

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The Cocos nucifera pollen, as one of the sources of allergen responsible for immediate hypersensitivity reaction, was confirmed by skin prick test, bronchial provocation test, and RAST. The whole pollen extract (WPE) of C. nucifera was fractionated by combination of gel filtration and ion-exchange columns with fast protein liquid chromatography (Pharmacia, Uppsala, Sweden). Three protein peaks designated Cocos II, Cocos VI, and Cocos VII exhibited allergenic properties, as tested by skin prick test, direct IgE ELISA, bronchial provocation test, and immunoblot analysis. In RAST inhibition, Cocos IIa (a high-molecular-weight protein) obtained by fractionation of Cocos II on Mono Q column (fast protein liquid chromatography) (Pharmacia) was found to be the most potent allergen in Cocos WPE, followed by Cocos VI and Cocos VII, which are low-molecular-weight proteins. The reference patterns of Cocos WPE on crossed immunoelectrophoresis and thin-layer isoelectric focusing were established for future standardization of Cocos WPE to be used in the diagnosis and immunotherapy of allergic patients.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:10661
Deposited On:04 Nov 2010 06:53
Last Modified:01 Jun 2011 12:05

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